《植物生理学报》 2010, 46(1): 42-46

柑橘叶片中叶黄素循环对 PSII 反应中心 D1 蛋白的保护效应

郑洁1,2, 周慧芳³, 陈蔚涛¹, 徐建旭², 郭延平^2,*

1 上海市柑橘研究所, 上海 201913; 2 浙江大学园艺系, 杭州 310029; 3 浙江省农业厅, 杭州 310020

通信作者：郭延平；E-mail: ypguo@zju.edu.cn；Tel: 0571-86971 121

摘　要：

用叶黄素循环抑制剂二硫苏糖醇(DTT)处理7 h的柑橘离体叶片, 其非光化学猝灭系数NPQ大幅度下降; 在中等强度光(500 μmol•m-2•s-1)和高强度光(1 500 μmol•m-2•s-1)下, DTT 处理的叶片光化学效率(F /F )分别下降 3.8% 和 39.7%, 光合电子传递vm速率(ETR)分别下降 12% 和 49.5%, D1 蛋白含量也分别下降 87% 和 92.3%; 黑暗对 DTT 处理叶片的各种荧光参数和 D1 蛋白的影响不大。显示叶黄素循环在保护光系统(PS) II 反应中心、抵御光抑制中有一定的积极效应, 可能影响了D1 蛋白周转。关键词: 柑橘; 叶黄素循环; 光系统II; 叶绿素荧光; D1 蛋白; 保护效应

关键词：2009-07-23

收稿：2009-07-23   修定：2009-11-23

资助：国家自然科学基金(30771497)。

Protective Effect of Xanthophyll Cycle on the D1 Protein of PSII in Leaves of Citrus reticulate Blanco.

ZHENG Jie^1,2, ZHOU Hui-Fang³, CHEN Wei-Tao¹, XU Jian-Xu², GUO Yan-Ping^2,*

¹Shanghai Citrus Institute, Shanghai 201913, China;²Department of Horticulture, Zhejiang University, Hangzhou 310029, China;³Department of Agriculture of Zhejiang Province, Hangzhou 310020, China

Corresponding author: GUO Yan-Ping; E-mail: ypguo@zju.edu.cn; Tel: 0571-86971 121

Abstract:

In this study, NPQ (non-photochemical quenching coefficient) in leaves of Citrus reticulate Blanco. declined significantly after seven hours with DTT (dithiothreitol) treatment. After DTT treatment, under moderate light of 500 μmol·m^-2·s^-1, Fv/Fm (photochemical efficiency), ETR (electron transport rate), and the content of D1 protein in leaves of C. reticulate decreased by 3.8%, 12%, and 87%, respectively. Under high light of 1 500 μmol·m^-2·s^-1, the corresponding parameters decreased by 39.7%, 49.5%, and 92.3%, respectively. In the dark, DTT treatment did not have significant effect on the chlorophyll fluorescence parameters and the level of the D1 protein. This result indicated that xanthophyll cycle played role in protecting PSII against photoinhibition and might have effect on the D1 protein turnover.

Key words: Citrus reticulate Blanco.; xanthophyll cycle; PSII; chlorophyll fluorescence; D1 protein; protective effect